Mapk1^tm1Moga
Targeted Allele Detail

Summary

• Symbol: Mapk1^tm1Moga
• Name: mitogen-activated protein kinase 1; targeted mutation 1, Masato Ogata
• MGI ID: MGI:3055889
• Synonyms: Erk2^floxS
• Gene: Mapk1 Location: Chr16:16801246-16865317 bp, + strand Genetic Position: Chr16, 10.53 cM
• Alliance: Mapk1^tm1Moga page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:89903
• Parent Cell Line: EB3/5 (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A targeting vector was used to insert a floxed region containing a splice acceptor, IRES, neo and SV40 pA into intron 2. Mapk1 cDNA with a polyA tail was inserted in place of exon 3. Protein was undetectable by immunoblot analysis. (J:89903)

Expression

In Mice Carrying this Mutation:

26 assay results

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Mapk1 Mutation: 43 strains or lines available

Notes

ES cell line EB3/5 is a mutant E14TG2a line carrying one inactivated allele of Pou5f1.
Rescue of placental defects by complementation with tetraploid embryos extends survival up to at least E13.5. These rescued mutant mice have a normal heart, exhibit normal placental development and grow as well as wild-type embryos.

References

• Original: J:89903 Hatano N, et al., Essential role for ERK2 mitogen-activated protein kinase in placental development. Genes Cells. 2003 Nov;8(11):847-56
• All: 3 reference(s)