Trp53^tm4Tyj
Targeted Allele Detail

Summary

• Symbol: Trp53^tm4Tyj
• Name: transformation related protein 53; targeted mutation 4, Tyler Jacks
• MGI ID: MGI:3056071
• Synonyms: S23A
• Gene: Trp53 Location: Chr11:69471185-69482699 bp, + strand Genetic Position: Chr11, 42.83 cM, cytoband B2-C
• Alliance: Trp53^tm4Tyj page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:92917
• Parent Cell Line: J1 (ES Cell)
• Strain of Origin: 129S4/SvJae

Mutation description

• Allele Type: Targeted (Not Specified)
• Mutation: Nucleotide substitutions
• Mutation details: Serine codon 23 (TCA) in exon 2 was changed to alanine (GCA) (p.S23A). Transient cre recombinase expression excised a loxP site flanked puromycin resistance gene cassette that was inserted into intron1. RT-PCR of mutant MEFs confirmed correct targeting. The mutation prevents the phosphorylation of the residue in the encoded peptide. (J:92917)

Tumor Data

List all tumor models in MMHCdb carrying Trp53^tm4Tyj

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Trp53 Mutation: 240 strains or lines available

References

• Original: J:92917 MacPherson D, et al., Defective apoptosis and B-cell lymphomas in mice with p53 point mutation at Ser 23. EMBO J. 2004 Sep 15;23(18):3689-99
• All: 5 reference(s)