Adgrg2tm1Bend
Targeted Allele Detail
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Symbol: |
Adgrg2tm1Bend |
Name: |
adhesion G protein-coupled receptor G2; targeted mutation 1, Ben Davies |
MGI ID: |
MGI:3056121 |
Synonyms: |
HE6- |
Gene: |
Adgrg2 Location: ChrX:159173686-159281066 bp, + strand Genetic Position: ChrX, 73.95 cM
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Alliance: |
Adgrg2tm1Bend page
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Germline Transmission: |
Earliest citation of germline transmission:
J:93019
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Parent Cell Line: |
E14TG2a (ES Cell)
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Strain of Origin: |
129P2/OlaHsd
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Allele Type: |
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Targeted (Null/knockout, Reporter) |
Mutations: |
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Insertion, Intragenic deletion
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Mutation details: A targeting vector was designed to replace exons 22 through 25 with a neomycin resistance gene, stop codons in all three reading frames, and an IRES-Beta-galactosidase cassette. The deleted sequence encoded the 7 transmembrane domain. Northern blot analysis of mutants indicated a lack of transcript for this domain. Two short, weakly expressed transcripts were present in mutants, one corresponding to exons 1-20 and the other corresponding to the truncated mRNA fused to Beta-gal. Ablation of wild-type protein was confirmed by Western blot of epididymal membrane samples, and lacZ activity was demonstrated by BLUO-GAL staining.
(J:93019)
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View phenotypes and curated references for all genotypes (concatenated display).
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Mouse strains and cell lines
available from the International Mouse Strain Resource
(IMSR) |
Carrying this Mutation: |
Mouse Strains: 0 strains available
Cell Lines: 0 lines available
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Carrying any Adgrg2 Mutation: |
13 strains or lines available
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Original: |
J:93019 Davies B, et al., Targeted deletion of the epididymal receptor HE6 results in fluid dysregulation and male infertility. Mol Cell Biol. 2004 Oct;24(19):8642-8 |
All: |
2 reference(s) |
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