Rad17^tm1Maas
Targeted Allele Detail

Summary

• Symbol: Rad17^tm1Maas
• Name: RAD17 checkpoint clamp loader component; targeted mutation 1, Alex Maas
• MGI ID: MGI:3056344
• Synonyms: mRad17^5'delta
• Gene: Rad17 Location: Chr13:100753672-100787559 bp, - strand Genetic Position: Chr13, 53.23 cM
• Alliance: Rad17^tm1Maas page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:93156
• Parent Cell Line: E14 (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A neo cassette was inserted into intron 2 in antisense orientation and a 110 bp segment of exon 3 was deleted resulting in a frameshift and formation of 3 stop codons via homologous recombination. RT-PCR showed the presence of two shorter transcripts and immunoblot results confirmed the absence of protein. (J:93156)

Expression

In Mice Carrying this Mutation:	4 assay results
In Structures Affected by this Mutation:	6 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Rad17 Mutation: 44 strains or lines available

References

• Original: J:93156 Budzowska M, et al., Mutation of the mouse Rad17 gene leads to embryonic lethality and reveals a role in DNA damage-dependent recombination. EMBO J. 2004 Sep 1;23(17):3548-58
• All: 1 reference(s)