Alcam^tm1Jawe
Targeted Allele Detail

Summary

• Symbol: Alcam^tm1Jawe
• Name: activated leukocyte cell adhesion molecule; targeted mutation 1, Joshua A Weiner
• MGI ID: MGI:3056579
• Synonyms: BEN^-
• Gene: Alcam Location: Chr16:52069359-52273444 bp, - strand Genetic Position: Chr16, 32.88 cM, cytoband B5
• Alliance: Alcam^tm1Jawe page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:93205
• Parent Cell Line: R1 (ES Cell)
• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutations: Insertion, Intragenic deletion
• Mutation details: Exon 1 was replaced by EGFP and loxP flanked neo via homologous recombination. Neo was removed via cre mediated recombination of the flanking loxP sites, resulting in increased GFP expression. Northern and immunohistochemical analysis confirmed the absence of RNA in adult brain and protein in E12 spinal cords. (J:93205)

Expression

In Structures Affected by this Mutation:

2 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 2 strains available Cell Lines: 0 lines available
• Carrying any Alcam Mutation: 39 strains or lines available

References

• Original: J:93205 Weiner JA, et al., Axon fasciculation defects and retinal dysplasias in mice lacking the immunoglobulin superfamily adhesion molecule BEN/ALCAM/SC1. Mol Cell Neurosci. 2004 Sep;27(1):59-69
• All: 18 reference(s)