Fn1^tm1Ksek
Targeted Allele Detail

Summary

• Symbol: Fn1^tm1Ksek
• Name: fibronectin 1; targeted mutation 1, Kiyotoshi Sekiguchi
• MGI ID: MGI:3056581
• Synonyms: EDB^-, EIIIB-null
• Gene: Fn1 Location: Chr1:71624679-71692359 bp, - strand Genetic Position: Chr1, 36.05 cM, cytoband C1-C5
• Alliance: Fn1^tm1Ksek page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:93250
• Parent Cell Line: E14.1 (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutation: Intragenic deletion
• Mutation details: Exon EDB (EIIIB) was deleted upon transient expression of Cre recombinase, which excised the floxed sequence containing a neomycin resistance gene and the exon. Immunoblot of mutant MEFs against an anti-EDB antibody indicated lack of protein from this exon. (J:93250)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Fn1 Mutation: 130 strains or lines available

References

• Original: J:93250 Fukuda T, et al., Mice lacking the EDB segment of fibronectin develop normally but exhibit reduced cell growth and fibronectin matrix assembly in vitro. Cancer Res. 2002 Nov 1;62(19):5603-10
• All: 3 reference(s)