Tg(ZP3)7812Dean
Transgene Detail

Summary

• Symbol: Tg(ZP3)7812Dean
• Name: transgene insertion 7812, Jurrien Dean
• MGI ID: MGI:3056745
• Synonyms: huZP3, TgN(HuZP3)3NIH
• Transgene: Tg(ZP3)7812Dean Location: unknown
• Alliance: Tg(ZP3)7812Dean page

Transgene origin

• Strain of Origin: FVB/N

Transgene description

• Transgene Type: Transgenic (Inserted expressed sequence)
• Mutation: Insertion
• Tg(ZP3)7812Dean expresses 1 gene
  Transgene expresses:
  

  Organism

  Expressed Gene

  Homolog in Mouse

  Note

  human

  ZP3 (7784)

  Zp3	(MGI:99215)

• Mutation details: This transgene comprises 6 kb of 5' flanking DNA from the mouse zona pellucida 3 gene ligated to the 5' half of the human zona pellucida 3 gene, including exons and introns 1-4, followed by cDNA encoded by exons 5-8 of the human gene and the bovine growth hormone polyadenylation signal. This transgenic line was estimated by Southern blot densitometry to carry two copies of the transgene. Transgene expression was shown by RNase protection analysis of multiple tissues to be ovary-specific. In situ hybridization localized expression to the cytoplasm of growing oocytes, at a level similar to that of the co-expressed endogenous mouse gene. Species-specific antibodies detect lower levels of human and mouse ZP3 in zonae pellucidae of mature transgenic oocytes than in human or wild-type mouse zonae, respectively; the total (human + mouse) approximating that in normal mouse zonae. The molecular mass of the transgenic protein suggests it undergoes post-translational modification similarly to the native human protein. (J:77459)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available

Notes

Three transgenic lines were established. This line and the line originally called TgN(HuZP3)2NIH each was estimated by Southern blot densitometry to carry 2 copies, and the line originally called TgN(HuZP3)1NIH to carry 4-6 copies of the transgene. Authors state that null-allele rescue results using the former two strains were identical.

References

• Original: J:77459 Rankin TL, et al., Human ZP3 restores fertility in Zp3 null mice without affecting order-specific sperm binding. Development. 1998 Jul;125(13):2415-24
• All: 3 reference(s)