Slc6a9^tm1.1Blt
Targeted Allele Detail

Summary

• Symbol: Slc6a9^tm1.1Blt
• Name: solute carrier family 6 (neurotransmitter transporter, glycine), member 9; targeted mutation 1.1, H Bluethmann
• MGI ID: MGI:3056923
• Synonyms: GlyT1tm1.1
• Gene: Slc6a9 Location: Chr4:117692240-117726502 bp, + strand Genetic Position: Chr4, 53.62 cM
• Alliance: Slc6a9^tm1.1Blt page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:93288
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: C57BL/6J

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: Transient Cre expression in embryonic stem cells whose endogenous gene bore previous modifications, including loxP sites flanking a 2000-base pair segment, resulted in deletion of exons 5 through 11, which encode most of the region common to all three receptor isoforms; this mutant allele also contains a previously introduced frt-flanked Pgk-neomycin phosphotransferase cassette in intron 11 and a deletion of 3.9 kb from intron 4. (J:93288)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Slc6a9 Mutation: 24 strains or lines available

Notes

Western blot analysis revealed that whole-brain protein extracts from adult heterozygous mutant mice contain approximately 50% the level of receptor protein in extracts from wild-type mice. While the pattern of immunohistologically-staining receptor distribution throughout the brain is the same in heterozygous and wild-type adult mice, the level of immunoreactive protein is significantly and proportionately reduced in all brain regions of the heterozygotes.

References

• Original: J:93288 Gabernet L, et al., Enhancement of the NMDA receptor function by reduction of glycine transporter-1 expression. Neurosci Lett. 2005 Jan 3;373(1):79-84
• All: 1 reference(s)