Zbtb20^{Tg(PDGFB-APPSwInd)20Lms}
Transgenic Allele Detail

Summary

• Symbol: Zbtb20^{Tg(PDGFB-APPSwInd)20Lms}
• Name: zinc finger and BTB domain containing 20; transgene insertion 20, Lennart Mucke
• MGI ID: MGI:3057148
• Synonyms: APP/J20, APP Tg, hAPP-J20, hAPPJ20, J20, PDAPP-J20, PDGF-APPSwInd, PDGF-hAPP695,751,770V171F, KM670/671NL, Tg(PDGFB-APP*Swe*Ind)20Lms
• Gene: Zbtb20 Location: Chr16:42728008-43462981 bp, + strand Genetic Position: Chr16, 28.44 cM, cytoband B4
• Alliance: Zbtb20^{Tg(PDGFB-APPSwInd)20Lms} page

Transgene origin

• Strain of Origin: (C57BL/6 x DBA/2)F2

Transgene description

• Transgene Type: Transgenic (Humanized sequence, Inserted expressed sequence)
• Mutations: Insertion, Intragenic deletion
• Zbtb20^{Tg(PDGFB-APPSwInd)20Lms} expresses 1 gene
  Transgene expresses:
  

  Organism

  Expressed Gene

  Homolog in Mouse

  Note

  human

  APP (351)

  App	(MGI:88059)

  The gene encodes for the Swedish and London mutations.

• Mutation details: The transgene expresses the mutant human amyloid protein precursor APPSwInd, which bears both the Swedish (K670N/M671L) and the Indiana (V717F) mutations, under the control of the human platelet derived growth factor, B polypeptide (PDGFB) promoter. Hemizygous transgenic mice express immunodetectable transgene product in cerebral neurons, with the highest level of expression occurring in the neocortex and hippocampus. ELISA analysis reveals approximate total amyloid beta peptides and 42 amino acid length amyloid beta peptides in neocortical and hippocampal tissue from transgenic mice. Line 20 inserted into an intron of the gene at 43086322-43127049 (Build GRCm38/mm10) resulting in a 40,727 bp deletion. Founder line 20 has a copy number of greater than 10. (J:62290, J:297213)

Disease models

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 1 line available
• Carrying any Zbtb20 Mutation: 58 strains or lines available

Notes

This line is also called line J20.

Diffuse amyloid beta peptides deposition forms in the dendate gyrus and neocortex at 5 to 7 months of age. Amyloid deposition is progressive, with all transgenic mice exhibiting plaques by age 8 to 10 months.

References

• Original: J:62290 Mucke L, et al., High-level neuronal expression of abeta 1-42 in wild-type human amyloid protein precursor transgenic mice: synaptotoxicity without plaque formation. J Neurosci. 2000 Jun 1;20(11):4050-8
• All: 276 reference(s)