Tg(UBC-GFP)30Scha
Transgene Detail

Summary

• Symbol: Tg(UBC-GFP)30Scha
• Name: transgene insertion 30, Brian Schaefer
• MGI ID: MGI:3057178
• Synonyms: Tg(UBCGFP)30Scha, UBC-GFP, Ub-GFP, UBI-EGFP, UBI-GFP, uGFP
• Transgene: Tg(UBC-GFP)30Scha Location: unknown Genetic Position: Chr17, Syntenic
• Alliance: Tg(UBC-GFP)30Scha page

Transgene origin

• Strain of Origin: C57BL/6

Transgene description

• Transgene Type: Transgenic (Reporter)
• Mutation: Insertion
• Mutation details: The transgene contains an enhanced green fluorescent protein open reading frame under the control of the human ubiquitin C promoter. The transgene integrated into chromosome 17 at nucleotide position 29,435,589 (reference sequence NC_000083.6; GRCm38.p6) - this is a noncoding region centromeric from the H-2 locus (~4.6 Mbp away from H-2 K1). (J:93490, J:242067, J:287278)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 9 strains available Cell Lines: 0 lines available

Notes

Homozygous transgenic mice are viable, fertile, normal in size, and do not display any gross physical or behavioral abnormalities. These mice express GFP in all tissues examined. Expression levels vary between certain hematapoietic cell types. GFP expression is uniform within a cell type lineage and remains constant throughout development. T cells have a 2-fold higher GFP expression than CD19+B220+ B cells or peripheral blood cells. Leukocytes and red blood cells from homozygous transgenic mice fluoresce at approximately twice the level of cells from hemizygous mice.

References

• Original: J:93490 Schaefer BC, et al., Observation of antigen-dependent CD8+ T-cell/ dendritic cell interactions in vivo. Cell Immunol. 2001 Dec 15;214(2):110-22
• All: 369 reference(s)