Tg(GFAP-APOE_i2)14Hol
Transgene Detail

Summary

• Symbol: Tg(GFAP-APOE_i2)14Hol
• Name: transgene insertion 14, David M Holtzman
• MGI ID: MGI:3057185
• Synonyms: GFAP-apoE2 line14, Tg(GFAP-APOE*2)14Hol, Tg(GFAP-APOE2)14Hol
• Transgene: Tg(GFAP-APOE_i2)14Hol Location: unknown
• Alliance: Tg(GFAP-APOE_i2)14Hol page

Transgene origin

• Strain of Origin: C57BL/6 and CBA

Transgene description

• Transgene Type: Transgenic (Humanized sequence, Inserted expressed sequence)
• Mutation: Insertion
• Tg(GFAP-APOE_i2)14Hol expresses 1 gene
  Transgene expresses:
  

  Organism

  Expressed Gene

  Homolog in Mouse

  Note

  human

  APOE (348)

  Apoe	(MGI:88057)

• Mutation details: The transgene contains sequence encoding human apolipoprotein E2 isoform under the control of the human glial fibrillary acidic protein (GFAP) promoter. (J:93487)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available

Notes

Transgenic mice do not express endogenous mouse apolipoprotein E (APOE). The expression pattern of the transgenic human apolipoprotein E2 isoform (APOE2) follows the endogenous mouse APOE and GFAP expression patterns in the brain. Human APOE2 is immunodetectable in glia and neuropil in developing and adult mutant mice. Cultured astrocytes from transgenic mice secrete APOE2 in lipoproteins that are similar in size to high density plasma lipoproteins (HDL). Detergent-soluble APOE2 protein levels in forebrain tissue from hemizygous mice and in adult human cortex tissue are similar.

Mice that are hemizygous or homozygous for the transgenic insert and homozygous for Apoe^tm1Unc are viable, normal in size, and do not display any gross physical or behavioral abnormalities.

References

• Original: J:93487 Sun Y, et al., Glial fibrillary acidic protein-apolipoprotein E (apoE) transgenic mice: astrocyte-specific expression and differing biological effects of astrocyte-secreted apoE3 and apoE4 lipoproteins. J Neurosci. 1998 May 1;18(9):3261-72
• All: 3 reference(s)