Gfi1^tm2Tmo
Targeted Allele Detail

Summary

• Symbol: Gfi1^tm2Tmo
• Name: growth factor independent 1 transcription repressor; targeted mutation 2, Tarik Moroy
• MGI ID: MGI:3057195
• Synonyms: Gfi^GFP, Gfi^KI
• Gene: Gfi1 Location: Chr5:107864521-107873671 bp, - strand Genetic Position: Chr5, 52.23 cM
• Alliance: Gfi1^tm2Tmo page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:93337
• Parent Cell Line: R1 (ES Cell)
• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A targeting vector was designed to replace exons 3-5 with an in-frame GFP and insert a floxed neo. Crossing with mice expressing Cre recombinase in the germ line removed the neomycin resistance gene. Fluorescence was detectible in virtually all thymocytes of mutant mice. Western blot showed that GFP expression in mutants was similar to that of gene expressed in wild-type mice. (J:93337)

Expression

In Mice Carrying this Mutation:

47 assay results

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Gfi1 Mutation: 31 strains or lines available

References

• Original: J:93337 Yucel R, et al., Gfi1:green fluorescent protein knock-in mutant reveals differential expression and autoregulation of the growth factor independence 1 (Gfi1) gene during lymphocyte development. J Biol Chem. 2004 Oct 24;279(39):40906-17
• All: 26 reference(s)