Tg(ZP2)2Dean
Transgene Detail

Summary

• Symbol: Tg(ZP2)2Dean
• Name: transgene insertion 2, Jurrien Dean
• MGI ID: MGI:3057275
• Synonyms: huZP2, TgN(huZP2)2Dean, TgN(HuZP2)2NIH
• Transgene: Tg(ZP2)2Dean Location: unknown
• Alliance: Tg(ZP2)2Dean page

Transgene origin

• Strain of Origin: FVB/N

Transgene description

• Transgene Type: Transgenic (Humanized sequence, Inserted expressed sequence)
• Mutation: Insertion
• Tg(ZP2)2Dean expresses 1 gene
  Transgene expresses:
  

  Organism

  Expressed Gene

  Homolog in Mouse

  Note

  human

  ZP2 (7783)

  Zp2	(MGI:99214)

• Mutation details: The transgene consists of a 16.3 kb human genomic DNA fragment containing all 19 exons of the zona pellucida 2 gene and 2.3 kb of 5' flanking DNA. Northern blot analysis and in situ hybridization of ovarian tissue demonstrated oocyte-specific transgene expression. Immunoblot analysis revealed coexpression of mouse and human ZP2 in oocytes. The molecular mass of the transgenic protein suggests it undergoes post-translational modification similarly to the native human protein. (J:93482)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available

Notes

3 transgenic lines, designated by the authors TgN(HuZP2)1NIH-3NIH, were generated. All three lines were used in experiments, and no distinction was made among their phenotypes.

References

• Original: J:93482 Rankin TL, et al., Fertility and taxon-specific sperm binding persist after replacement of mouse sperm receptors with human homologs. Dev Cell. 2003 Jul;5(1):33-43
• All: 1 reference(s)