Nhlh2^tm1Thbr
Targeted Allele Detail

Summary

• Symbol: Nhlh2^tm1Thbr
• Name: nescient helix loop helix 2; targeted mutation 1, Thomas Braun
• MGI ID: MGI:3057284
• Synonyms: NSCL-2^-, NSCL-2^lacZ
• Gene: Nhlh2 Location: Chr3:101917425-101922808 bp, + strand Genetic Position: Chr3, 44.3 cM
• Alliance: Nhlh2^tm1Thbr page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:93303
• Parent Cell Line: J1 (ES Cell)
• Strain of Origin: 129S4/SvJae

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutations: Insertion, Intragenic deletion
• Mutation details: An IRES-lacZ-neomycin resistance cassette replaced the first and part of the second exon including parts of the DNA binding bHLH region, and placed lacZ under control of endogenous transcriptional elements. Beta-galactosidase was expresssed under the control of the endogenous promoter. (J:93303)

Expression

In Mice Carrying this Mutation:	20 assay results
In Structures Affected by this Mutation:	4 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Nhlh2 Mutation: 13 strains or lines available

References

• Original: J:93303 Kruger M, et al., NSCL-1 and NSCL-2 synergistically determine the fate of GnRH-1 neurons and control necdin gene expression. EMBO J. 2004 Nov 27;23(21):4353-64
• All: 5 reference(s)