Trp53^tm1Adv
Targeted Allele Detail

Summary

• Symbol: Trp53^tm1Adv
• Name: transformation related protein 53; targeted mutation 1, Annemieke de Vries
• MGI ID: MGI:3057305
• Synonyms: p53.S389A
• Gene: Trp53 Location: Chr11:69471185-69482699 bp, + strand Genetic Position: Chr11, 42.83 cM, cytoband B2-C
• Alliance: Trp53^tm1Adv page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:93328
• Parent Cell Line: J1 (ES Cell)
• Strain of Origin: 129S4/SvJae

Mutation description

• Allele Type: Targeted (Not Specified)
• Mutation: Nucleotide substitutions
• Mutation details: A targeting vector was designed to introduce a p.S389A mutation (T>G) in exon 11. This should abolish phosphorylation of the protein at that amino acid residue in the encoded peptide. A loxP site flanked neomycin resistance gene cassette was introduced downstream of exon 11 and subsequently excised by crossing with Cre expressing deleter mice. Expression of the mutated allele was confirmed by RT-PCR analysis. (J:93328)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Trp53 Mutation: 240 strains or lines available

References

• Original: J:93328 Bruins W, et al., Increased Sensitivity to UV Radiation in Mice with a p53 Point Mutation at Ser389. Mol Cell Biol. 2004 Nov;24(20):8884-94
• All: 4 reference(s)