Ereg^tm1Dwt
Targeted Allele Detail

Summary

• Symbol: Ereg^tm1Dwt
• Name: epiregulin; targeted mutation 1, David W Threadgill
• MGI ID: MGI:3057313
• Gene: Ereg Location: Chr5:91222481-91241505 bp, + strand Genetic Position: Chr5, 44.78 cM
• Alliance: Ereg^tm1Dwt page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:93327
• Parent Cell Line: TL1/TL-1 (ES Cell)
• Strain of Origin: 129S6/SvEvTac

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutation: Insertion
• Mutation details: A targeting vector was designed to introduce a nuclear localization signal-tagged lacZ, SV40pA and neo gene into exon 1, immediately upstream of the ATG. PCR revealed a transcript from the PGK promoter, however, Western blot detected a frameshift mutation that rendered the protein nonfunctional. No wild-type transcript or protein was observed in RT-PCR or Western blot of mutants. (J:93327)

Expression

In Structures Affected by this Mutation:

2 anatomical structure(s)

Tumor Data

List all tumor models in MMHCdb carrying Ereg^tm1Dwt

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Ereg Mutation: 18 strains or lines available

References

• Original: J:93327 Lee D, et al., Epiregulin is not essential for development of intestinal tumors but is required for protection from intestinal damage. Mol Cell Biol. 2004 Nov;24(20):8907-16
• All: 8 reference(s)