Ret^{tm3.1(RET)Jmi}
Targeted Allele Detail

Summary

• Symbol: Ret^{tm3.1(RET)Jmi}
• Name: ret proto-oncogene; targeted mutation 3.1, Jeffrey Milbrandt
• MGI ID: MGI:3057333
• Synonyms: Ret^DN, RetDN
• Gene: Ret Location: Chr6:118128706-118174679 bp, - strand Genetic Position: Chr6, 55.86 cM, cytoband E3-F1
• Alliance: Ret^{tm3.1(RET)Jmi} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:93262
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: Not Specified

Mutation description

• Allele Type: Targeted (Dominant negative)
• Mutation: Insertion
• Mutation details: Human RET9 cDNA with Y1062F and L985P mutations and a floxed neo were inserted into exon 1 of the endogenous locus. Leucine 985 was at the end of the kinase domain. Mating with mice expressing Cre in the germline excised the neo. RT-PCR showed expression of the mutant allele in brain. (J:93262)

Expression

In Mice Carrying this Mutation:	4 assay results
In Structures Affected by this Mutation:	7 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Ret Mutation: 54 strains or lines available

References

• Original: J:93262 Jain S, et al., Mice expressing a dominant-negative Ret mutation phenocopy human Hirschsprung disease and delineate a direct role of Ret in spermatogenesis. Development. 2004 Dec;131(21):5503-13
• All: 3 reference(s)