Pax3^tm1Mrc
Targeted Allele Detail

Summary

• Symbol: Pax3^tm1Mrc
• Name: paired box 3; targeted mutation 1, Mario R Capecchi
• MGI ID: MGI:3487369
• Synonyms: Pax3:Fkhr, Pax3:Foxo1, Pax3^P3Fm
• Gene: Pax3 Location: Chr1:78077904-78173771 bp, - strand Genetic Position: Chr1, 39.79 cM
• Alliance: Pax3^tm1Mrc page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:93444
• Parent Cell Line: R1 (ES Cell)
• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Mutation description

• Allele Type: Targeted (Inserted expressed sequence, Reporter)
• Mutation: Insertion
• Mutation details: A targeting vector was inserted into the 3' region of the gene, resulting in loxP sites flanking exons 8-10 as well as an inserted polyA/stop signal from the C2 gene and exons 2 and 3 of the Foxo1 gene (with an IRES-eYFP cassette in exon 3). Crossing with FLP expressing mice excised an FRT flanked neo that was also inserted downstream. The mutation allowed normal functioning until activation of Cre recombinase. Cre causes the fusion of exons 1-7 of Pax3 to a region of Foxo1 containing exons 2 and 3, as well as 6.5 kb of the UTR and genomic sequence. Simultaneously, an IRES-eYFP marker is expressed. (J:93444)

Disease models

Tumor Data

List all tumor models in MMHCdb carrying Pax3^tm1Mrc

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Pax3 Mutation: 50 strains or lines available

References

• Original: J:93444 Keller C, et al., Alveolar rhabdomyosarcomas in conditional Pax3:Fkhr mice: cooperativity of Ink4a/ARF and Trp53 loss of function. Genes Dev. 2004 Dec 1;18(21):2614-26
• All: 15 reference(s)