Parp1^tm2Zqw
Targeted Allele Detail

Summary

• Symbol: Parp1^tm2Zqw
• Name: poly (ADP-ribose) polymerase family, member 1; targeted mutation 2, Zhao-Qi Wang
• MGI ID: MGI:3497740
• Synonyms: PARP-1^KI
• Gene: Parp1 Location: Chr1:180396456-180428564 bp, + strand Genetic Position: Chr1, 84.44 cM, cytoband H5
• Alliance: Parp1^tm2Zqw page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:93479
• Parent Cell Line: E14.1 (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted (Not Specified)
• Mutation: Nucleotide substitutions
• Mutation details: A point mutation in exon 5 was introduced to create an Asp214Asn substitution that would render the protein resistant to caspase cleavage. Transient expression of Cre recombinase excised a neo included in the targeting vector. Western blot showed an equal amount of protein in mutant and wild-type fibroblasts, thymocytes and macrophages. A second Western blot analysis confirmed the mutant protein's resistance to caspase cleaveage during apoptosis in thymocytes. The point mutation did not affect protein expression or activity in response to DNA damage. (J:93479)

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Parp1 Mutation: 66 strains or lines available

References

• Original: J:93479 Petrilli V, et al., Noncleavable poly(ADP-ribose) polymerase-1 regulates the inflammation response in mice. J Clin Invest. 2004 Nov;114(8):1072-81
• All: 3 reference(s)