Ap3m2^tm1Ohno
Targeted Allele Detail

Summary

• Symbol: Ap3m2^tm1Ohno
• Name: adaptor-related protein complex 3, mu 2 subunit; targeted mutation 1, Hiroshi Ohno
• MGI ID: MGI:3497921
• Synonyms: mu3Bdeltaneo, mu3B-KO
• Gene: Ap3m2 Location: Chr8:23277370-23295638 bp, - strand Genetic Position: Chr8, 11.42 cM
• Alliance: Ap3m2^tm1Ohno page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:93312
• Parent Cell Line: E14.1 (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A targeting vector was designed to replace downstream of the ATG for mu3B in exon 2 with an EGFP and a floxed neo. Cre expression excised the neo. RT-PCR indicated the absence of transcript in mutant brain and spinal cord. EGFP was detected in neurons throughout the brain. (J:93312)

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Ap3m2 Mutation: 24 strains or lines available

References

• Original: J:93312 Nakatsu F, et al., Defective function of GABA-containing synaptic vesicles in mice lacking the AP-3B clathrin adaptor. J Cell Biol. 2004 Oct 25;167(2):293-302
• All: 4 reference(s)