Ap3m2tm1Ohno
Targeted Allele Detail
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Symbol: |
Ap3m2tm1Ohno |
Name: |
adaptor-related protein complex 3, mu 2 subunit; targeted mutation 1, Hiroshi Ohno |
MGI ID: |
MGI:3497921 |
Synonyms: |
mu3Bdeltaneo, mu3B-KO |
Gene: |
Ap3m2 Location: Chr8:23277370-23295638 bp, - strand Genetic Position: Chr8, 11.42 cM
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Alliance: |
Ap3m2tm1Ohno page
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Germline Transmission: |
Earliest citation of germline transmission:
J:93312
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Parent Cell Line: |
E14.1 (ES Cell)
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Strain of Origin: |
129P2/OlaHsd
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Allele Type: |
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Targeted (Null/knockout) |
Mutations: |
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Insertion, Intragenic deletion
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Mutation details: A targeting vector was designed to replace downstream of the ATG for mu3B in exon 2 with an EGFP and a floxed neo. Cre expression excised the neo. RT-PCR indicated the absence of transcript in mutant brain and spinal cord. EGFP was detected in neurons throughout the brain.
(J:93312)
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Key: |
hm |
homozygous |
ht |
heterozygous |
tg |
involves transgenes |
√ |
phenotype observed |
cn |
conditional genotype |
cx |
complex: > 1 genome feature |
ot |
other: hemizygous, indeterminate,... |
N |
normal phenotype |
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Genotype/ Background: |
| Allelic Composition | Genetic Background | Cell Line(s) |
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Loading... | | | involves: 129P2/OlaHsd * C57BL/6 | |
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Phenotypes: |
Affected Systems |
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behavior/neurological
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seizures
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increased susceptibility to pharmacologically induced seizures
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abnormal kindling response
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nonconvulsive seizures
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nervous system
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seizures
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√
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increased susceptibility to pharmacologically induced seizures
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√
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abnormal kindling response
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√
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nonconvulsive seizures
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√
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abnormal hippocampus morphology
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abnormal synaptic vesicle morphology
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abnormal nervous system electrophysiology
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enhanced long-term potentiation
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√
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decreased neurotransmitter release
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√
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View phenotypes and curated references for all genotypes (concatenated display).
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Mouse strains and cell lines
available from the International Mouse Strain Resource
(IMSR) |
Carrying this Mutation: |
Mouse Strains: 0 strains available
Cell Lines: 0 lines available
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Carrying any Ap3m2 Mutation: |
24 strains or lines available
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Original: |
J:93312 Nakatsu F, et al., Defective function of GABA-containing synaptic vesicles in mice lacking the AP-3B clathrin adaptor. J Cell Biol. 2004 Oct 25;167(2):293-302 |
All: |
4 reference(s) |
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