Tg(AZIP/F)1Vsn
Transgene Detail

Summary

• Symbol: Tg(AZIP/F)1Vsn
• Name: transgene insertion 1, Charles Vinson
• MGI ID: MGI:3497939
• Synonyms: A-ZIP/F-1, A-ZIP/F1
• Transgene: Tg(AZIP/F)1Vsn Location: unknown
• Alliance: Tg(AZIP/F)1Vsn page

Transgene origin

• Strain of Origin: FVB/N

Transgene description

• Transgene Type: Transgenic (Dominant negative, Inserted expressed sequence)
• Mutation: Insertion
• Mutation details: A 7.6 kb segment of the Fabp4 enhancer/promoter was used to drive expression of an artificially constructed cDNA encoding a dominant-negative protein to inhibit the DNA binding of both Cebpa and Jun family members. The protein is 104 amino acids long, with an amino-terminal 9 amino acid Flag epitope, a 13 amino acid linker, a 21 amino acid designed acidic amphipathic helix and a 61 amino acid designed leucine zipper (ZIP/F). The transgene also had an SV40 intron and a PolyA signal. (J:50807)

Expression

In Structures Affected by this Mutation:

8 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available

Notes

Phenotypic Similarity to Human Syndrome: lipoatrophic diabetes (Seip-Berardinelli syndrome) J:50807, J:226956.

References

• Original: J:50807 Moitra J, et al., Life without white fat: a transgenic mouse. Genes Dev. 1998 Oct 15;12(20):3168-81
• All: 35 reference(s)