Ido1^tm1Alm
Targeted Allele Detail

Summary

• Symbol: Ido1^tm1Alm
• Name: indoleamine 2,3-dioxygenase 1; targeted mutation 1, Andrew L Mellor
• MGI ID: MGI:3505776
• Synonyms: IDO^-, Ido1^-, Ido1-KO
• Gene: Ido1 Location: Chr8:25074148-25086987 bp, - strand Genetic Position: Chr8, 12.76 cM
• Alliance: Ido1^tm1Alm page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:93796
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: 129X1/SvJ

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A Betagal and neomycin cassette replaced exons 3-5, which encode critical portions of the enzyme catalytic site. Also, a translation stop codon was introduced into exon 2. RT-PCR of total epididymis RNA from mutants showed no transcript was present. Western blot of mutant epididymis confirmed a lack of protein. (J:93797)

Expression

In Mice Carrying this Mutation:	1 RNA-Seq or microarray experiment(s)
In Structures Affected by this Mutation:	3 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 2 strains available Cell Lines: 0 lines available
• Carrying any Ido1 Mutation: 29 strains or lines available

References

• Original: J:93796 Mellor AL, et al., Cutting edge: induced indoleamine 2,3 dioxygenase expression in dendritic cell subsets suppresses T cell clonal expansion. J Immunol. 2003 Aug 15;171(4):1652-5
• All: 139 reference(s)