Mospd3^{Gt(PT1-ATG)124Wrst}
Gene trapped Allele Detail

Summary

• Symbol: Mospd3^{Gt(PT1-ATG)124Wrst}
• Name: motile sperm domain containing 3; gene trap 124, Wolfgang Wurst
• MGI ID: MGI:3510668
• Synonyms: Mospd3^{Gt(PT1-ATG)1Lmf}, Mospd3^GTR124For
• Gene: Mospd3 Location: Chr5:137594907-137599320 bp, - strand Genetic Position: Chr5, 76.59 cM, cytoband Telomere
• Alliance: Mospd3^{Gt(PT1-ATG)124Wrst} page

Mutation origin

• Mutant Cell Line: R124
• Germline Transmission: Earliest citation of germline transmission: J:93956
• Parent Cell Line: R1 (ES Cell)
• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Mutation description

• Allele Type: Gene trapped (Null/knockout, Reporter)
• Mutation: Insertion of gene trap vector Vector Type: gene trap
• Mutation details: The gene trap vector PT1-ATG inserted in to a 5' exon in the reverse orientation. The vector contained the En2 splice acceptor site, lacZ, and PGK-neo. RNase protection analysis indicated that the integrated vector was expressed as a fusion protein, including 320 bp of Mospd3 combined with 23 bp of En2 intronic and 120 bp of En2 exonic sequence. (J:31729, J:47966)

Expression

In Structures Affected by this Mutation:

6 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Mospd3 Mutation: 19 strains or lines available

References

• Original: J:47966 McClive P, et al., Gene trap integrations expressed in the developing heart: insertion site affects splicing of the PT1-ATG vector. Dev Dyn. 1998 Jun;212(2):267-76
• All: 3 reference(s)