Lat^tm2.1Mal
Targeted Allele Detail

Summary

• Symbol: Lat^tm2.1Mal
• Name: linker for activation of T cells; targeted mutation 2.1, Bernard Malissen
• MGI ID: MGI:3510778
• Synonyms: Lat3YF, Lat^Y7/8/9F
• Gene: Lat Location: Chr7:125962996-125968742 bp, - strand Genetic Position: Chr7, 69.01 cM
• Alliance: Lat^tm2.1Mal page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:94074
• Parent Cell Line: CK35 (ES Cell)
• Strain of Origin: 129S2/SvPas

Mutation description

• Allele Type: Targeted
• Mutation: Nucleotide substitutions
• Mutation details: Tyrosine residues at positions 175, 195 and 235 (p.Y175F, p.Y195F, p.Y235F), in exons 9-11, respectively, were mutated to phenylalanine residues. Western blot shows that the amount of protein in mutant thymocytes is similar to that of wild-type. A floxed neo was inserted between exons 9 and 10 and subsequently removed by crossing with deleter mice. (J:94074)

Expression

In Structures Affected by this Mutation:

7 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Lat Mutation: 10 strains or lines available

References

• Original: J:94074 Nunez-Cruz S, et al., LAT regulates gammadelta T cell homeostasis and differentiation. Nat Immunol. 2003 Oct;4(10):999-1008
• All: 6 reference(s)