Chrna4^tm2Lst
Targeted Allele Detail

Summary

• Symbol: Chrna4^tm2Lst
• Name: cholinergic receptor, nicotinic, alpha polypeptide 4; targeted mutation 2, Henry A Lester
• MGI ID: MGI:3511287
• Synonyms: L9'A, Leu9'Ala
• Gene: Chrna4 Location: Chr2:180664104-180685339 bp, - strand Genetic Position: Chr2, 103.54 cM
• Alliance: Chrna4^tm2Lst page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:93873
• Parent Cell Line: CJ7 (ES Cell)
• Strain of Origin: 129S1/Sv-Oca2⁺ Tyr⁺ Kitl⁺

Mutation description

• Allele Type: Targeted (Not Specified)
• Mutations: Insertion, Single point mutation
• Mutation details: A point mutation was introduced to change a leucine to alanine within the putative pore-forming M2 domain. A loxP flanked neo cassette was also inserted in the construct. Correctly targeted ES cells were transfected with Cre plasmid to remove the neo. (J:93873)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 2 strains available Cell Lines: 0 lines available
• Carrying any Chrna4 Mutation: 44 strains or lines available

References

• Original: J:93873 Tapper AR, et al., Nicotine activation of alpha4* receptors: sufficient for reward, tolerance, and sensitization. Science. 2004 Nov 5;306(5698):1029-32
• All: 6 reference(s)