Myh9^{Gt(Betageo-btk)1Dash}
Gene trapped Allele Detail

Summary

• Symbol: Myh9^{Gt(Betageo-btk)1Dash}
• Name: myosin, heavy polypeptide 9, non-muscle; gene trap 1, Tadashi Matsushita
• MGI ID: MGI:3511350
• Synonyms: MYH9^-
• Gene: Myh9 Location: Chr15:77644788-77726315 bp, - strand Genetic Position: Chr15, 36.81 cM
• Alliance: Myh9^{Gt(Betageo-btk)1Dash} page

Mutation origin

• Mutant Cell Line: OST208974
• Germline Transmission: Earliest citation of germline transmission: J:94067
• Parent Cell Line: Lex-1 (ES Cell)
• Strain of Origin: 129S5/SvEvBrd

Mutation description

• Allele Type: Gene trapped (Null/knockout, Reporter)
• Mutation: Insertion of gene trap vector
• Mutation details: A gene trap vector containing a splice acceptor sequence, promotorless Betageo, pA, PGK, exon 1 from Btk and splice donor sequence, was inserted into intron 1 of the endogenous gene. Btk contained a stop codon in each reading frame to prevent translation of downstream fusion transcripts. Protein expression was reduced by half in heterozygotes as shown by immonoblot analysis. (J:94067)
• Sequence Tags: Sequence tag details (1 tag)

  Tag ID	GenBank ID	Method	Tag Location (trapped strand)*	Select
  OST208974	CG575326 ; (MGI Seq Detail)	3' RACE	Chr15:77692067-77697432 bp (-)
  * GRCm39; (strand of gene trap mutagenesis)

• Genome Context: Genome Browser view of this mutation;

  279 gene trap insertions have trapped this gene; View all gene trap sequence tags in this region

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Myh9 Mutation: 219 strains or lines available

References

• Original: J:94067 Matsushita T, et al., Targeted disruption of mouse ortholog of the human MYH9 responsible for macrothrombocytopenia with different organ involvement: hematological, nephrological, and otological studies of heterozygous KO mice. Biochem Biophys Res Commun. 2004 Dec 24;325(4):1163-71
• All: 2 reference(s)