Snap25^tm2Mcw
Targeted Allele Detail

Summary

• Symbol: Snap25^tm2Mcw
• Name: synaptosomal-associated protein 25; targeted mutation 2, Michael C Wilson
• MGI ID: MGI:3511662
• Synonyms: Snap25^Tkneo
• Gene: Snap25 Location: Chr2:136555373-136624348 bp, + strand Genetic Position: Chr2, 67.56 cM
• Alliance: Snap25^tm2Mcw page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:94005
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: Not Specified

Mutation description

• Allele Type: Targeted (Not Applicable)
• Mutation: Insertion
• Mutation details: A Tkneo selection gene flanked by loxP sites inserted in between the tandemly arranged exons 5a and 5b and exon 6 via homologous recombination, disrupting the developmental regulation of alternative splicing between exons 5a and 5b. In homozygous mutants, RT-PCR confirmed that expression of the two isoforms is altered such that the expression of the a isoform is increased and the b isoform is decreased. (J:94005)

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Snap25 Mutation: 26 strains or lines available

References

• Original: J:94005 Bark C, et al., Developmentally regulated switch in alternatively spliced SNAP-25 isoforms alters facilitation of synaptic transmission. J Neurosci. 2004 Oct 6;24(40):8796-805
• All: 2 reference(s)