Car3^tm1Gkim
Targeted Allele Detail

Summary

• Symbol: Car3^tm1Gkim
• Name: carbonic anhydrase 3; targeted mutation 1, Geumsoo Kim
• MGI ID: MGI:3511866
• Gene: Car3 Location: Chr3:14928598-14937441 bp, + strand Genetic Position: Chr3, 3.22 cM, cytoband A2
• Alliance: Car3^tm1Gkim page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:94222
• Parent Cell Line: TC1/TC-1 (ES Cell)
• Strain of Origin: 129S6/SvEvTac

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: The targeting event replaced 4.2 kb of DNA containing exons 3, 4 and 5 with a neomycin resistance (PGK-neo ) cassette. Northern and western blot analyses verified that mRNA and protein encoded by the targeted gene are both absent in tissues of homozygous mutant mice. Microarray analysis revealed a small (~30% +/- 10%) but statistically significant increase in Car13 transcript levels in mice lacking CAR3. (J:94222)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 2 strains available Cell Lines: 0 lines available
• Carrying any Car3 Mutation: 18 strains or lines available

References

• Original: J:94222 Kim G, et al., Carbonic anhydrase III is not required in the mouse for normal growth, development, and life span. Mol Cell Biol. 2004 Nov;24(22):9942-7
• All: 5 reference(s)