Tg(MMTV-rtTA)1Lach
Transgene Detail

Summary

• Symbol: Tg(MMTV-rtTA)1Lach
• Name: transgene insertion 1, Lewis A Chodosh
• MGI ID: MGI:3511875
• Synonyms: MMTV-rtTA, MMTV-rtTA-pA, MTB, Tg(rtTa)1Lach
• Transgene: Tg(MMTV-rtTA)1Lach Location: unknown
• Alliance: Tg(MMTV-rtTA)1Lach page

Transgene origin

• Strain of Origin: FVB/N

Transgene description

• Transgene Type: Transgenic (Transactivator)
• Mutation: Insertion
• Mutation details: The transgene comprises a 2.9-kb DNA fragment containing the MMTV LTR, 1.2 kb of LTR-upstream sequence, and 0.6 kb of v-Hras leader sequence; a 1.0-kb fragment containing a minigene encoding full-length reverse tetracycline transactivator (rtTA: "Tet-On"); and a 1.8 kb fragment containing the splicing and polyadenylation signals from SV40. Northern blot analysis readily detected rtTA transcripts in total mammary gland RNA from 5 week-old, doxycycline-treated, female transgenic mice. Upon induction by doxycycline, this transgene promoted high-level, homogeneous, reversible expression of beta-galactosidase or luciferase reporter transgenes in mammary epithelium, including terminal end buds, of female mice of various ages and reproductive states; expression was higher in pregnant and lactating mice than in virgin or post-involutional females. Except in older females continuously induced for at least 10 weeks, mammary glands of these mice were morphologically and functionally normal. rtTA and/or reporter expression was observed at lower levels in salivary gland, seminal vesicle and thymus, again only upon doxycycline administration. Older (15 weeks) induced bitrangenic female mice expressed beta-galactosidase in only 15-20% of mammary epithelial cells; however, levels of rtTA mRNA were only slightly lower than in 5 week-old induced mice. (J:75048)

Disease models

Tumor Data

List all tumor models in MMHCdb carrying Tg(MMTV-rtTA)1Lach

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available

References

• Original: J:75048 Gunther EJ, et al., A novel doxycycline-inducible system for the transgenic analysis of mammary gland biology. FASEB J. 2002 Mar;16(3):283-92
• All: 111 reference(s)