Sufu^tm1Aeb
Targeted Allele Detail

Summary

• Symbol: Sufu^tm1Aeb
• Name: SUFU negative regulator of hedgehog signaling; targeted mutation 1, Allen E Bale
• MGI ID: MGI:3512046
• Gene: Sufu Location: Chr19:46385335-46477243 bp, + strand Genetic Position: Chr19, 38.85 cM
• Alliance: Sufu^tm1Aeb page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:101731
• Parent Cell Line: W9.5/W95 (ES Cell)
• Strain of Origin: 129S1/Sv-Oca2⁺ Tyr⁺ Kitl⁺

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: Vector contains exon 4 through the 3' end of intron 6 then PGK-driven NEO in orientation reverse to the gene followed by the 3' end of exon 7 through the end of exon 8. Correct targeting replaces the intron 6/exon 7 splice site and a portion of exon 7 with the neo casette. Northern and Western blot analysis confirmed the absence of transcription and translation. (J:101731)

Expression

In Structures Affected by this Mutation:

2 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Sufu Mutation: 59 strains or lines available

References

• Original: J:101731 Cooper AF, et al., Cardiac and CNS defects in a mouse with targeted disruption of suppressor of fused. Development. 2005 Oct;132(19):4407-17
• All: 1 reference(s)