Tpst2^tm1Klm
Targeted Allele Detail

Summary

• Symbol: Tpst2^tm1Klm
• Name: protein-tyrosine sulfotransferase 2; targeted mutation 1, Kevin L Moore
• MGI ID: MGI:3512111
• Gene: Tpst2 Location: Chr5:112424557-112463227 bp, + strand Genetic Position: Chr5, 54.65 cM
• Alliance: Tpst2^tm1Klm page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:107617
• Parent Cell Line: AB2.2 (ES Cell)
• Strain of Origin: 129S7/SvEvBrd-Hprt1^b-m2

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A 0.88 kb fragment spanning the start codon and the remainder of exon 3 was replaced by a 1.7 kb PGKneo cassette. A 4.5 kb EcoRI-ApaI genomic fragment was used as the left arm for homologous recombination and was cloned into the pPGKneo vector upstream of the PGKneo cassette. Northern blot analysis and QPCR confirmed the absence of transcription. (J:107617)

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Tpst2 Mutation: 106 strains or lines available

References

• Original: J:107617 Borghei A, et al., Targeted disruption of tyrosylprotein sulfotransferase-2, an enzyme that catalyzes post-translational protein tyrosine O-sulfation, causes male infertility. J Biol Chem. 2006 Apr 7;281(14):9423-31
• All: 9 reference(s)