Rr169^tm2Rao
Targeted Allele Detail

Summary

• Symbol: Rr169^tm2Rao
• Name: regulatory region 169; targeted mutation 2, Anjana Rao
• MGI ID: MGI:3512141
• Synonyms: HS IV^-, Il4^tm2Rao
• Gene: Rr169 Location: Chr11:53499081-53501004 bp Genetic Position: Chr11, Syntenic
• Alliance: Rr169^tm2Rao page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:94039
• Parent Cell Line: TC1/TC-1 (ES Cell)
• Strain of Origin: 129S6/SvEvTac

Mutation description

• Allele Type: Targeted (Modified regulatory region, Null/knockout)
• Mutation: Disruption caused by insertion of vector
• Mutation details: A loxP site flanked neomycin resistance gene cassette replaced a 1.9 kb section containing DNase I hypersensitivity site IV (HS IV), an Il4 silencer located between Il4 and Kif3a. Transient Cre recombinase expression removed the neo cassette in ES cells. DNase hypersensitivity analysis of mutant cells showed a loss of HS IV, but no disruption of the other hypersensitivity sites. (J:94039)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Rr169 Mutation: 0 strains or lines available

References

• Original: J:94039 Ansel KM, et al., Deletion of a conserved Il4 silencer impairs T helper type 1-mediated immunity. Nat Immunol. 2004 Dec;5(12):1251-9
• All: 2 reference(s)