Fgf2^tm2Doe
Targeted Allele Detail

Summary

• Symbol: Fgf2^tm2Doe
• Name: fibroblast growth factor 2; targeted mutation 2, Thomas Doetschman
• MGI ID: MGI:3512180
• Synonyms: 18-kDa FGF2KO, Fgf2 lmw ko
• Gene: Fgf2 Location: Chr3:37402616-37464255 bp, + strand Genetic Position: Chr3, 18.41 cM, cytoband A2-B
• Alliance: Fgf2^tm2Doe page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:94550
• Parent Cell Line: E14TG2a (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: Exchange reaction of a Tag & Exchange targeting scheme 4 bp alteration without introduction of marker gene or loxP or frt sites. To produce this ko allele, the Hprt minigene in the tagged Fgf2 ko allele (Zhou et al, Nature Medicine, 4:201, 1998) was "exchanged" by gene targeting with a mutant exon 1 with the sequence CTGCAG replacing the wild-type CCATGC. The mutant sequence encodes an alanine GCA in place of the methionine ATG translational start site of the 18-kDa low molecular weight isoform. The adjacent upstream wobble base was also changed from C to T so that the composite of these three base changes generated a PstI site that is diagnostic for the exchanged allele. Cells with the exchanged allele were selected for the loss of HPRT function using 5 mg/mL 6-thioguanine. There are no LoxP, Frt or marker gene sequences in this new allele. (J:94550)

Expression

In Structures Affected by this Mutation:

3 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Fgf2 Mutation: 19 strains or lines available

Notes

E14TG2a congenic line with one Fgf2 ko allele was used to produce this allele.

References

• Original: J:94550 Garmy-Susini B, et al., Role of fibroblast growth factor-2 isoforms in the effect of estradiol on endothelial cell migration and proliferation. Circ Res. 2004 May 28;94(10):1301-9
• All: 11 reference(s)