About   Help   FAQ
Fgf2tm2Doe
Targeted Allele Detail
Summary
Symbol: Fgf2tm2Doe
Name: fibroblast growth factor 2; targeted mutation 2, Thomas Doetschman
MGI ID: MGI:3512180
Synonyms: 18-kDa FGF2KO, Fgf2 lmw ko
Gene: Fgf2  Location: Chr3:37402616-37464255 bp, + strand  Genetic Position: Chr3, 18.41 cM, cytoband A2-B
Alliance: Fgf2tm2Doe page
Mutation
origin
Germline Transmission:  Earliest citation of germline transmission: J:94550
Parent Cell Line:  E14TG2a (ES Cell)
Strain of Origin:  129P2/OlaHsd
Mutation
description
Allele Type:    Targeted (Null/knockout)
Mutations:    Insertion, Intragenic deletion
 
Mutation detailsExchange reaction of a Tag & Exchange targeting scheme 4 bp alteration without introduction of marker gene or loxP or frt sites. To produce this ko allele, the Hprt minigene in the tagged Fgf2 ko allele (Zhou et al, Nature Medicine, 4:201, 1998) was "exchanged" by gene targeting with a mutant exon 1 with the sequence CTGCAG replacing the wild-type CCATGC. The mutant sequence encodes an alanine GCA in place of the methionine ATG translational start site of the 18-kDa low molecular weight isoform. The adjacent upstream wobble base was also changed from C to T so that the composite of these three base changes generated a PstI site that is diagnostic for the exchanged allele. Cells with the exchanged allele were selected for the loss of HPRT function using 5 mg/mL 6-thioguanine. There are no LoxP, Frt or marker gene sequences in this new allele. (J:94550)
Phenotypes
Loading...
View phenotypes and curated references for all genotypes (concatenated display).
Expression
In Structures Affected by this Mutation: 3 anatomical structure(s)
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 1 strain available      Cell Lines: 0 lines available
Carrying any Fgf2 Mutation:  19 strains or lines available
Notes
E14TG2a congenic line with one Fgf2 ko allele was used to produce this allele.
References
Original:  J:94550 Garmy-Susini B, et al., Role of fibroblast growth factor-2 isoforms in the effect of estradiol on endothelial cell migration and proliferation. Circ Res. 2004 May 28;94(10):1301-9
All:  12 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
Citing These Resources
Funding Information
Warranty Disclaimer, Privacy Notice, Licensing, & Copyright
Send questions and comments to User Support.
last database update
11/12/2024
MGI 6.24
The Jackson Laboratory