Gt(ROSA)26Sor^{tm1(Akt1/EGFP)Glas}
Targeted Allele Detail

Summary

• Symbol: Gt(ROSA)26Sor^{tm1(Akt1/EGFP)Glas}
• Name: gene trap ROSA 26, Philippe Soriano; targeted mutation 1, David J Glass
• MGI ID: MGI:3512295
• Synonyms: Akt^Ind.Tg, c.a.Akt-EGFP
• Gene: Gt(ROSA)26Sor Location: Chr6:113044389-113054205 bp, - strand Genetic Position: Chr6, 52.73 cM
• Alliance: Gt(ROSA)26Sor^{tm1(Akt1/EGFP)Glas} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:94134
• Parent Cell Line: R1 (ES Cell)
• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Mutation description

• Allele Type: Targeted (Conditional ready, Constitutively active, Inserted expressed sequence, Reporter)
• Mutation: Insertion
• Gt(ROSA)26Sor^{tm1(Akt1/EGFP)Glas} expresses 1 gene
  Knock-in expresses:
  

  Organism	Expressed Gene	Note
  mouse	Akt1 (MGI:87986)

• Mutation details: The HSA-driven CreERt2 transgene was inserted in cis with a cassette containing a constitutively active form of Atk1 fused to EGFP (c.a.Akt-EGFP). Prior to the addition of tamoxifen, the HSA promoter drives transctiption of CreERt2, with absence of c.a.Akt-EGFP transcription. Upon the addition of tamoxifen, CreERt2 expression would drive its own excision, leaving the c.a.Akt-EGFP cassette downstream of the HSA promoter. (J:94134)

Expression

In Structures Affected by this Mutation:

3 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Gt(ROSA)26Sor Mutation: 993 strains or lines available

References

• Original: J:94134 Lai KM, et al., Conditional activation of akt in adult skeletal muscle induces rapid hypertrophy. Mol Cell Biol. 2004 Nov;24(21):9295-304
• All: 1 reference(s)