Pcdh12^tm1Hub
Targeted Allele Detail

Summary

• Symbol: Pcdh12^tm1Hub
• Name: protocadherin 12; targeted mutation 1, Philippe Huber
• MGI ID: MGI:3512625
• Synonyms: Pcdh12^-
• Gene: Pcdh12 Location: Chr18:38400145-38417454 bp, - strand Genetic Position: Chr18, 20.17 cM, cytoband B3
• Alliance: Pcdh12^tm1Hub page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:94272
• Parent Cell Line: AT1 (ES Cell)
• Strain of Origin: 129S2/SvPasCrl

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: 1.85 kb of the gene including the initiation codon and 1689 bp of the 5' coding sequence was replaced with a promoterless LacZ-PGK-neo. Mutant mice failed to produce LacZ expression, either due to a mislocalization of the reporter gene downstream of the promoter region or the removal of important regulatory regions after recombination. Immunoblot of protein extracts from H5V cells and placenta from mutants demonstrated a lack of product from three out of four epitope peptides. (J:94272)

Expression

In Mice Carrying this Mutation:

1 RNA-Seq or microarray experiment(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Pcdh12 Mutation: 40 strains or lines available

References

• Original: J:94272 Rampon C, et al., Protocadherin 12 (VE-cadherin 2) is expressed in endothelial, trophoblast, and mesangial cells. Exp Cell Res. 2005 Jan 1;302(1):48-60
• All: 3 reference(s)