Sox10^tm2(rtTA)Weg
Targeted Allele Detail

Summary

• Symbol: Sox10^tm2(rtTA)Weg
• Name: SRY (sex determining region Y)-box 10; targeted mutation 2, Michael Wegner
• MGI ID: MGI:3513104
• Synonyms: Sox10^rtTA
• Gene: Sox10 Location: Chr15:79039113-79048690 bp, - strand Genetic Position: Chr15, 37.7 cM
• Alliance: Sox10^tm2(rtTA)Weg page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:94207
• Parent Cell Line: E14.1 (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted (Null/knockout, Transactivator)
• Mutation: Insertion
• Mutation details: Exons 3-5 (the entire coding region) were replaced with a reverse tetracycline controlled transactivator (rtTA) gene and a loxP-flanked Neo cassette, placing the start codon of the rtTA at the position of the endogenous start codon in exon 3, via homologous recombination. The Neo cassette was removed by crossing to mice expressing germline Cre-recombinase. Crossing to mice expressing lacZ under the control of the tetracycline inducible promoter and then treating with doxycycline resulted in X-gal staining (indicating rtTA activity) that recapitulated Sox10 expression. (J:94207)

Expression

In Structures Affected by this Mutation:

2 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Sox10 Mutation: 33 strains or lines available

References

• Original: J:94207 Ludwig A, et al., Sox10-rtTA mouse line for tetracycline-inducible expression of transgenes in neural crest cells and oligodendrocytes. Genesis. 2004 Nov;40(3):171-5
• All: 13 reference(s)