Prg4^tm1Mawa
Targeted Allele Detail

Summary

• Symbol: Prg4^tm1Mawa
• Name: proteoglycan 4 (megakaryocyte stimulating factor, articular superficial zone protein); targeted mutation 1, Matthew Warman
• MGI ID: MGI:3513195
• Synonyms: Prg4^-
• Gene: Prg4 Location: Chr1:150325163-150341916 bp, - strand Genetic Position: Chr1, 63.84 cM, cytoband G
• Alliance: Prg4^tm1Mawa page

Prg4^tm1Mawa/Prg4^tm1Mawa mice develop joint disease with increasing age

Show the 3 phenotype image(s) involving this allele.

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:96756
• Parent Cell Line: R1 (ES Cell)
• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: The targeting construct was designed to create a Prg4-B-gal fusion protein by substituting lacZ for exon 6. Northern blot and in situ hybridization, however, detected no transcript in livers of mutant mice. RT-PCR of synovial and liver mRNA showed aberrant mRNA splicing from the allele which was thought to create a nonsense mediated mRNA decay. (J:96756)

Expression

In Structures Affected by this Mutation:

5 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Prg4 Mutation: 71 strains or lines available

References

• Original: J:96756 Rhee DK, et al., The secreted glycoprotein lubricin protects cartilage surfaces and inhibits synovial cell overgrowth. J Clin Invest. 2005 Mar;115(3):622-31
• All: 19 reference(s)