Brca1^tm3Cxd
Targeted Allele Detail

Summary

• Symbol: Brca1^tm3Cxd
• Name: breast cancer 1, early onset; targeted mutation 3, Chu-Xia Deng
• MGI ID: MGI:3513253
• Synonyms: Brca1^S971A
• Gene: Brca1 Location: Chr11:101379590-101442781 bp, - strand Genetic Position: Chr11, 65.18 cM, cytoband D
• Alliance: Brca1^tm3Cxd page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:94114
• Parent Cell Line: TC1/TC-1 (ES Cell)
• Strain of Origin: 129S6/SvEvTac

Mutation description

• Allele Type: Targeted (Inserted expressed sequence)
• Mutation: Insertion
• Mutation details: A serine to alanine substitution at amino acid 971 (S971A) was introduced into exon 11 and a floxed neo cassette upstream of exon 11 via homologous recombination. Mating with mice expressing cre recombinase in the germ line excised the floxed neo. This allele results in a mutation of the Chek2 phosphorylation site. RT-PCR showed that transcript is expressed at a level comparable to wild-type. (J:94114)

Tumor Data

List all tumor models in MMHCdb carrying Brca1^tm3Cxd

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Brca1 Mutation: 114 strains or lines available

References

• Original: J:94114 Kim SS, et al., Uterus hyperplasia and increased carcinogen-induced tumorigenesis in mice carrying a targeted mutation of the Chk2 phosphorylation site in Brca1. Mol Cell Biol. 2004 Nov;24(21):9498-507
• All: 1 reference(s)