Slc7a1^tm1Rbmn
Targeted Allele Detail

Summary

• Symbol: Slc7a1^tm1Rbmn
• Name: solute carrier family 7 (cationic amino acid transporter, y+ system), member 1; targeted mutation 1, Robert A Bosselman
• MGI ID: MGI:3513834
• Synonyms: mCAT-1^-
• Gene: Slc7a1 Location: Chr5:148264220-148336714 bp, - strand Genetic Position: Chr5, 88.48 cM
• Alliance: Slc7a1^tm1Rbmn page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:39649
• Parent Cell Line: Other (see notes) (ES Cell)
• Strain of Origin: 129

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A PGK-neo replaced exon 4 by homologous recombination. Exon 4 encodes the YGE residues required for virus binding. Successful disruption of the gene was confirmed by XC fusion assay and mRNA was negative for amino acid transport activity. (J:39649)

Expression

In Structures Affected by this Mutation:

2 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Slc7a1 Mutation: 94 strains or lines available

Notes

ES cell line = E14K (129P2/OlaHsd) or W9.5 (129S1/Sv-p⁺ Tyr⁺ Kitl⁺).

References

• Original: J:39649 Perkins CP, et al., Anemia and perinatal death result from loss of the murine ecotropic retrovirus receptor mCAT-1. Genes Dev. 1997 Apr 1;11(7):914-25
• All: 1 reference(s)