Nde1^tm1Caw
Targeted Allele Detail

Summary

• Symbol: Nde1^tm1Caw
• Name: nudE neurodevelopment protein 1; targeted mutation 1, Christopher A Walsh
• MGI ID: MGI:3514026
• Synonyms: Nde1^-
• Gene: Nde1 Location: Chr16:13981139-14010792 bp, + strand Genetic Position: Chr16, 9.7 cM
• Alliance: Nde1^tm1Caw page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:94756
• Parent Cell Line: J1 (ES Cell)
• Strain of Origin: 129S4/SvJae

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutation: Intragenic deletion
• Mutation details: A loxP site was inserted upstream of exon 2 and a floxed PGK-neomycin cassette was inserted downstream. Transient transfection of properly targeted ES cells with a Cre-expressing plasmid resulted in excision of the selection cassette together with exon 2. Immunoblot analysis confirmed the absence of protein product. (J:94756)

Expression

In Structures Affected by this Mutation:

2 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Nde1 Mutation: 32 strains or lines available

References

• Original: J:94756 Feng Y, et al., Mitotic spindle regulation by Nde1 controls cerebral cortical size. Neuron. 2004 Oct 14;44(2):279-93
• All: 8 reference(s)