Matr3^{Gt(RRR075)Byg}
Gene trapped Allele Detail

Summary

• Symbol: Matr3^{Gt(RRR075)Byg}
• Name: matrin 3; gene trap RRR075, BayGenomics
• MGI ID: MGI:3514145
• Synonyms: Matr3^Gt-ex13, Matr3^{Gt(pGT0Lxf)1Ucd}, Matr3^{Gt(pGTOLxf)ex13Rlm}
• Gene: Matr3 Location: Chr18:35695191-35726888 bp, + strand Genetic Position: Chr18, 19.14 cM, cytoband C
• Alliance: Matr3^{Gt(RRR075)Byg} page

Mutation origin

• Mutant Cell Line: RRR075
• Germline Transmission: Earliest citation of germline transmission: J:94077
• Parent Cell Line: Other (see notes) (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Gene trapped (Null/knockout)
• Mutation: Insertion of gene trap vector Vector: pGT0Lxf Vector Type: gene trap
• Mutation details: The beta-geo cassette inserted into exon 13. 3' RACE analysis of mouse E14.5 brain and heart tissues showed that the long 3' RACE product (the only form detected in brain) is reduced in heterozygous brain, and that both long and short 3' RACE products are reduced in heterozygous heart. While interruption of the 3' end by the gene trap insertion reduced wild-type transcripts in heterozygotes, this was accompanied by the expression of a fusion transcript that may encode a functional or partly functional fusion protein. Western blot analyses of heterozygous heart and brain failed to detect a significant alteration in protein levels but did reveal the presence of the Matrin 3-beta-geo fusion protein in embryonic heart.
• Sequence Tags: Sequence tag details (1 tag)

  Tag ID	GenBank ID	Method	Tag Location (trapped strand)*	Select
  RRR075	CG784970 ; (MGI Seq Detail)	5' RACE	Chr18:35717606-35717926 bp (+)
  * GRCm39; (strand of gene trap mutagenesis)

• Genome Context: Genome Browser view of this mutation;

  10 gene trap insertions have trapped this gene; View all gene trap sequence tags in this region

Expression

In Mice Carrying this Mutation:	27 assay results
In Structures Affected by this Mutation:	7 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Matr3 Mutation: 112 strains or lines available

Notes

All BayGenomics gene trap mutations were generated in either CGR8 or E14TG2a (129P2/OlaHsd) parental ES cell lines, with the majority in subline E14TG2a.4. The specific ES cell line in which each mutation was made is not specified.

References

• Original: J:94077 Mutant Mouse Regional Resource Centers, Information obtained from the Mutant Mouse Regional Resource Centers (MMRRC). Unpublished. 2004-2015
• All: 3 reference(s)