Dmbt1^tm1Kumc
Targeted Allele Detail

Summary

• Symbol: Dmbt1^tm1Kumc
• Name: deleted in malignant brain tumors 1; targeted mutation 1, Robert C De Lisle
• MGI ID: MGI:3514149
• Gene: Dmbt1 Location: Chr7:130633787-130723357 bp, + strand Genetic Position: Chr7, 73.2 cM, cytoband F4
• Alliance: Dmbt1^tm1Kumc page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:132597
• Parent Cell Line: RW-4 (ES Cell)
• Strain of Origin: 129X1/SvJ

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: The first exon and 300 bp of the first intron were replaced by a cassette composed of a GFP cassette inserted downstream of the TATA box in such a way that the GFP start codon replaced that of the endogenous gene as well as a PGK neomycin gene in reverse orientation. Lack of expression was confirmed by QRT-PCR and Western blot analysis. No GFP protein expression or mRNA was detected. (J:132597)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Dmbt1 Mutation: 111 strains or lines available

References

• Original: J:132597 De Lisle RC, et al., Effects of Muclin (Dmbt1) deficiency on the gastrointestinal system. Am J Physiol Gastrointest Liver Physiol. 2008 Mar;294(3):G717-27
• All: 3 reference(s)