Notch1^tm3(cre)Rko
Targeted Allele Detail

Summary

• Symbol: Notch1^tm3(cre)Rko
• Name: notch 1; targeted mutation 3, Raphael Kopan
• MGI ID: MGI:3514150
• Synonyms: N1::cre, N1::cre^LO, N1::cre^tg, N1IP-Cre, N1IP::CRE^LOW, NIP-Cre
• Gene: Notch1 Location: Chr2:26347914-26393834 bp, - strand Genetic Position: Chr2, 18.91 cM
• Alliance: Notch1^tm3(cre)Rko page

Corneal plaques and increased angiogenesis in the ear of Notch1^tm2Rko/Notch1^tm3(cre)Rko mice

Show the 6 phenotype image(s) involving this allele.

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:119910
• Parent Cell Line: RW-4 (ES Cell)
• Strain of Origin: 129X1/SvJ

Mutation description

• Allele Type: Targeted (Recombinase)
• Mutation: Insertion
• Notch1^tm3(cre)Rko expression driven by 1 gene
  Knock-in expression driven by:
  

  Organism	Driver Gene	Note
  mouse	Notch1 (MGI:97363)

• The intracellular domain was replaced with cre recombinase. (J:119910)

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	Notch1 (mouse) Summary of all recombinase alleles driven by Notch1. Comparative matrix view of recombinase activities Alliance mouse Notch1 gene page

Phenotypes

Key:

hm	homozygous	ht	heterozygous	tg	involves transgenes	√	phenotype observed
cn	conditional genotype	cx	complex: > 1 genome feature	ot	other: hemizygous, indeterminate,...	N	normal phenotype

Genotype/
Background:

	Allelic Composition	Genetic Background	Cell Line(s)
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hm1	Notch1^tm3(cre)Rko/Notch1^tm3(cre)Rko	involves: 129X1/SvJ * C57BL/6J * SJL
cn2	Notch1^tm2Rko/Notch1^tm3(cre)Rko	involves: 129X1/SvJ * C57BL/6

Phenotypes:

Affected Systems	hm1	cn2
show or hide all annotated terms
cardiovascular system		√
dilated liver sinusoidal space		√
increased angiogenesis		√
hemoperitoneum		√
cornea vascularization		√
growth/size/body		√
enlarged spleen		√
hematopoietic system		√
extramedullary hematopoiesis		√
abnormal spleen morphology		√
enlarged spleen		√
immune system		√
abnormal spleen morphology		√
enlarged spleen		√
chronic liver inflammation		√
liver/biliary system		√
chronic liver inflammation		√
abnormal liver morphology		√
abnormal hepatic cord morphology		√
abnormal liver parenchyma morphology		√
abnormal liver lobule morphology		√
dilated liver sinusoidal space		√
pale liver		√
mortality/aging	√	√
premature death		√
embryonic lethality during organogenesis, complete penetrance	√
neoplasm		√
increased hemangioma incidence		√
vision/eye		√
abnormal cornea morphology		√
cornea vascularization		√
cornea deposits		√

View phenotypes and curated references for all genotypes (concatenated display).

Expression

In Structures Affected by this Mutation:

7 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Notch1 Mutation: 118 strains or lines available

References

• Original: J:119910 Vooijs M, et al., Mapping the consequence of Notch1 proteolysis in vivo with NIP-CRE. Development. 2007 Feb;134(3):535-44
• All: 14 reference(s)