Pck1^tm1Rwh
Targeted Allele Detail

Summary

• Symbol: Pck1^tm1Rwh
• Name: phosphoenolpyruvate carboxykinase 1, cytosolic; targeted mutation 1, Richard W Hanson
• MGI ID: MGI:3514160
• Gene: Pck1 Location: Chr2:172994866-173001067 bp, + strand Genetic Position: Chr2, 95.79 cM, cytoband C1-qter
• Alliance: Pck1^tm1Rwh page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:145210
• Parent Cell Line: E14 (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A PGK-BetaGal-neomycin resistance cassette was inserted so that it deleted a region of the gene from -569 in the promoter to a point in the 5' end of exon 3. No detectable mRNA was found in the liver and kidney. No enzyme activity was found in the liver. (J:145210)

Expression

In Structures Affected by this Mutation:

2 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Pck1 Mutation: 47 strains or lines available

References

• Original: J:145210 Hakimi P, et al., Phosphoenolpyruvate carboxykinase and the critical role of cataplerosis in the control of hepatic metabolism. Nutr Metab (Lond). 2005;2:33
• All: 1 reference(s)