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Apbb1tm1Quhu
Targeted Allele Detail
Summary
Symbol: Apbb1tm1Quhu
Name: amyloid beta precursor protein binding family B member 1; targeted mutation 1, Qubai Hu
MGI ID: MGI:3514266
Synonyms: p97FE65-
Gene: Apbb1  Location: Chr7:105207690-105230860 bp, - strand  Genetic Position: Chr7, 55.9 cM
Alliance: Apbb1tm1Quhu page
Mutation
origin
Germline Transmission:  Earliest citation of germline transmission: J:94887
Parent Cell Line:  R1 (ES Cell)
Strain of Origin:  (129X1/SvJ x 129S1/Sv)F1-Kitl+
Mutation
description
Allele Type:    Targeted (Null/knockout)
Mutations:    Insertion, Intragenic deletion
 
Mutation detailsA phosphoglycerate kinase (PGK) promoter-driven neomycin cassette replaced most of the exon 2 region. Northern and Western blot analysis as well as immunohistochemistry confirmed the absence of full-length (2.6 kB) transcription and 97kD translation products. However, the expression of a shorter 60kD isoform was retained in mutant mice, and this isoform appeared to be translated from an alternatively spliced trancript that did not contain exon 2. Western and GST pull-down analysis of brain lysates demonstrated that the expression of the 60kD isoform was increased 5-fold in mutant mice. (J:94887)
Phenotypes
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View phenotypes and curated references for all genotypes (concatenated display).
Expression
In Mice Carrying this Mutation: 1 RNA-Seq or microarray experiment(s)
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 1 strain available      Cell Lines: 0 lines available
Carrying any Apbb1 Mutation:  52 strains or lines available
References
Original:  J:94887 Wang B, et al., Isoform-specific knockout of FE65 leads to impaired learning and memory. J Neurosci Res. 2004 Jan 1;75(1):12-24
All:  6 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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last database update
10/29/2024
MGI 6.24
The Jackson Laboratory