Apbb1^tm1Quhu
Targeted Allele Detail

Summary

• Symbol: Apbb1^tm1Quhu
• Name: amyloid beta precursor protein binding family B member 1; targeted mutation 1, Qubai Hu
• MGI ID: MGI:3514266
• Synonyms: p97FE65^-
• Gene: Apbb1 Location: Chr7:105207690-105230860 bp, - strand Genetic Position: Chr7, 55.9 cM
• Alliance: Apbb1^tm1Quhu page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:94887
• Parent Cell Line: R1 (ES Cell)
• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A phosphoglycerate kinase (PGK) promoter-driven neomycin cassette replaced most of the exon 2 region. Northern and Western blot analysis as well as immunohistochemistry confirmed the absence of full-length (2.6 kB) transcription and 97kD translation products. However, the expression of a shorter 60kD isoform was retained in mutant mice, and this isoform appeared to be translated from an alternatively spliced trancript that did not contain exon 2. Western and GST pull-down analysis of brain lysates demonstrated that the expression of the 60kD isoform was increased 5-fold in mutant mice. (J:94887)

Expression

In Mice Carrying this Mutation:

1 RNA-Seq or microarray experiment(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Apbb1 Mutation: 52 strains or lines available

References

• Original: J:94887 Wang B, et al., Isoform-specific knockout of FE65 leads to impaired learning and memory. J Neurosci Res. 2004 Jan 1;75(1):12-24
• All: 6 reference(s)