Tg(Ttr-Foxo1)305Dac
Transgene Detail

Summary

• Symbol: Tg(Ttr-Foxo1)305Dac
• Name: transgene insertion 305, Domenico Accili
• MGI ID: MGI:3521642
• Transgene: Tg(Ttr-Foxo1)305Dac Location: unknown
• Alliance: Tg(Ttr-Foxo1)305Dac page

Transgene origin

• Strain of Origin: Not Applicable

Transgene description

• Transgene Type: Transgenic (Inserted expressed sequence)
• Mutation: Insertion
• Tg(Ttr-Foxo1)305Dac expresses 1 gene
  Transgene expresses:
  

  Organism	Expressed Gene	Note
  mouse	Foxo1 (MGI:1890077)

• Mutation details: This transgene uses a Ttr promoter to drive expression of a c-Myc tagged Foxo1, mutated to include a single amino acid substitution replacing the phosphorylation site Ser253 with a non-phosphorylatable amino acid. This line expressed the fusion protein in liver at levels approximately threefold higher than line 307. Expression was also shown in Beta-cell and duct-cells of the pancreas by RT-PCR and immunohistochemistry. Total Foxo1 expression in islets was determined to be fivefold higher in this line than in line 307 or wild-type mice by RT-PCR. (J:79560)

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available

Notes

Microinjected into FVB x B6 fertilized eggs.

References

• Original: J:79560 Nakae J, et al., Regulation of insulin action and pancreatic beta-cell function by mutated alleles of the gene encoding forkhead transcription factor Foxo1. Nat Genet. 2002 Oct;32(2):245-53
• All: 4 reference(s)