Tg(Ttr-Foxo1)307Dac
Transgene Detail

Summary

• Symbol: Tg(Ttr-Foxo1)307Dac
• Name: transgene insertion 307, Domenico Accili
• MGI ID: MGI:3521643
• Synonyms: Foxo1^S253A
• Transgene: Tg(Ttr-Foxo1)307Dac Location: unknown
• Alliance: Tg(Ttr-Foxo1)307Dac page

Transgene origin

• Strain of Origin: Not Applicable

Transgene description

• Transgene Type: Transgenic (Inserted expressed sequence)
• Mutation: Insertion
• Tg(Ttr-Foxo1)307Dac expresses 1 gene
  Transgene expresses:
  

  Organism	Expressed Gene	Note
  mouse	Foxo1 (MGI:1890077)

• Mutation details: This transgene uses a Ttr promoter to drive expression of a c-Myc tagged Foxo1, mutated to include a single amino acid substitution replacing the phosphorylation site Ser253 with a non-phosphorylatable amino acid. This line expressed the fusion protein in liver at levels approximately threefold lower than line 305. Total Foxo1 expression in islets was determined to be fivefold lower in this line than in line 305 by RT-PCR. No expression was found in islets. (J:79560)

Expression

In Structures Affected by this Mutation:

2 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available

Notes

Microinjected into FVB x B6 fertilized eggs.

References

• Original: J:79560 Nakae J, et al., Regulation of insulin action and pancreatic beta-cell function by mutated alleles of the gene encoding forkhead transcription factor Foxo1. Nat Genet. 2002 Oct;32(2):245-53
• All: 7 reference(s)