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Uba7tm1Dez
Targeted Allele Detail
Summary
Symbol: Uba7tm1Dez
Name: ubiquitin-like modifier activating enzyme 7; targeted mutation 1, Dong-Er Zhang
MGI ID: MGI:3521787
Synonyms: Uba7tm1Dzh, Ube1l-, Ube1L-
Gene: Uba7  Location: Chr9:107852766-107861255 bp, + strand  Genetic Position: Chr9, 59.07 cM
Alliance: Uba7tm1Dez page
Mutation
origin
Germline Transmission:  Earliest citation of germline transmission: J:144149
Parent Cell Line:  Not Specified (ES Cell)
Strain of Origin:  129
Mutation
description
Allele Type:    Targeted (Conditional ready, No functional change)
Mutation:    Insertion
 
Mutation detailsExon 14 encodes the active site for this enzyme that is responsible for activating ISG15 conjugation activity. LoxP sites were inserted upstream of exon 14 and downstream of exon 18. A Frt- and loxP- flanked neomycin resistance cassette was also inserted downstream of exon 18. Correct targeting was confirmed by Southern blotting. Splenocytes and thymocytes from homozygotes that were treated with IFN-beta expressed a larger mRNA transcript than controls due to the neomycin insertion. Tissue extracts from homozygote mice failed to demonstrate conjugating activity between ISG15 and larger protein complexes (i.e. no ISGylation activity). (J:144149)
Phenotypes
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View phenotypes and curated references for all genotypes (concatenated display).
Tumor Data
List all tumor models in MMHCdb carrying Uba7tm1Dez
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 0 strains available      Cell Lines: 0 lines available
Carrying any Uba7 Mutation:  36 strains or lines available
References
Original:  J:144149 Kim KI, et al., Ube1L and protein ISGylation are not essential for alpha/beta interferon signaling. Mol Cell Biol. 2006 Jan;26(2):472-9
All:  12 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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last database update
10/29/2024
MGI 6.24
The Jackson Laboratory