Rock1^tm1Leiw
Targeted Allele Detail

Summary

• Symbol: Rock1^tm1Leiw
• Name: Rho-associated coiled-coil containing protein kinase 1; targeted mutation 1, Lei Wei
• MGI ID: MGI:3521801
• Gene: Rock1 Location: Chr18:10064401-10182045 bp, - strand Genetic Position: Chr18, 4.91 cM, cytoband A2
• Alliance: Rock1^tm1Leiw page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:111469
• Parent Cell Line: AB2.2 (ES Cell)
• Strain of Origin: 129S7/SvEvBrd-Hprt1^b-m2

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutation: Insertion
• Mutation details: The coding sequence of beta-galactosidase followed by a PGK-neo cassette was inserted in frame downstream of the sequence for residue 180. QRT-PCR and Western blot analysis confirmed the absence of transcription and translation products. (J:111469)

Expression

In Structures Affected by this Mutation:

2 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Rock1 Mutation: 60 strains or lines available

References

• Original: J:111469 Zhang YM, et al., Targeted deletion of ROCK1 protects the heart against pressure overload by inhibiting reactive fibrosis. FASEB J. 2006 May;20(7):916-25
• All: 17 reference(s)