Gucy2d^tm1Sdm
Targeted Allele Detail

Summary

• Symbol: Gucy2d^tm1Sdm
• Name: guanylate cyclase 2d; targeted mutation 1, Steven D Munger
• MGI ID: MGI:3521821
• Synonyms: Gucy2d^-
• Gene: Gucy2d Location: Chr7:98089623-98126685 bp, + strand Genetic Position: Chr7, 53.86 cM
• Alliance: Gucy2d^tm1Sdm page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:124965
• Parent Cell Line: R1 (ES Cell)
• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutation: Insertion
• Mutation details: An IRES-Mapt-lacZ reporter with a floxed neo cassette was inserted between exon 2 and 3. RT-PCR confirmed the lack of transcription from the null allele. LacZ expression was confirmed by immunohistochemistry in a small number of main olfactory epithelium consistent with the small number of Gucy2d+ neurons previously reported. Cre-mediated recombination removed the floxed neo cassette. (J:124965)

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Gucy2d Mutation: 43 strains or lines available

References

• Original: J:124965 Leinders-Zufall T, et al., Contribution of the receptor guanylyl cyclase GC-D to chemosensory function in the olfactory epithelium. Proc Natl Acad Sci U S A. 2007 Sep 4;104(36):14507-12
• All: 5 reference(s)